Device

Part:BBa_M50073

Designed by: Melat Birbo and Charlotte Philp   Group: Stanford BIOE44 - S11   (2017-06-10)


E. coli pH sensor

Both of these plasmid constructs were inserted into E. coli to sense an overall pH range of 4.8-8. This device fluoresces turquoise fluorescent protein based on a promoter that is pH sensitive for pHs of 5.5-8. This device fluoresces red fluorescent protein based on a promoter that is pH sensitive for pHs of 4.8-7.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1747
    Illegal AgeI site found at 1859
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None